Human Myosin Light Chain 2, Regulatory, Cardiac (MYL2) ELISA Kit

SKU: orb776154

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 2, Regulatory, Cardiac(MYL2).

Research Area

Cardiovascular Research, Metabolism Research, Musculoskeletal & Connective Tissue Research

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYL2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	7.82-500 pg/mL
Sensitivity	3.2 pg/mL
Concentration	500 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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CMH10 ELISA Kit, MLC2 ELISA Kit, Myosin,Light Chain 2,Regulatory,Cardiac,Slow ELISA Kit, Myosin regulatory light chain 2, ventricular/cardiac muscle isoform ELISA Kit

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Human Myosin Light Chain 2, Regulatory, Cardiac (MYL2) ELISA Kit

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UniProt

P10916

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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