Recombinant Human Nuclear Antigen Antibody

SKU: orb2637336

Description

This antibody recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.

Research Area

Immunology & Inflammation

Images & Validation

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Tested Applications	FACS, IF
Dilution Range	Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-3ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Human Nuclear Antigen antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG2a, kappa
Clone No.	r235-1
Immunogen	Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for the recombinant Human Nuclear Antigen antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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SDS-PAGE analysis of purified, BSA-free recombinant Human Nuclear Antigen antibody (r235-1) as confirmation of integrity and purity.

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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Immunofluorescence

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