Human Osteogenic Growth Peptide (OGP) ELISA Kit

SKU: orb779064

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Osteogenic Growth Peptide(OGP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Osteogenic Growth Peptide(OGP).

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OGP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OGP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OGP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type	Competitive
Assay Time	2.5h
Range	15.63-1000 pg/mL
Sensitivity	4.69 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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OGP

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Human Osteogenic Growth Peptide (OGP) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (1)

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Human (1)

Xin Mu 1, Yikun Yang 2, Huachang Zhang 1, Hao Song 1, Yiwei Li 1, Chuanfei Wei 1, Li Xu 1, Keyi Li 3, Kun Liu 3, Fabin Han 1, Chao Liu 4, Nan Zhang Sequentially Releasing Aspirin and Osteogenic Growth Peptide Scaffold Modulates Immunity and Bone Homeostasis to Enhance Periodontal Bone Regeneration ACS Appl Mater Interfaces, (2025)

PubMed: 40471700

Applications

ELISA

Reactivity

Human