Human OTUB2 protein

SKU: orb392226

Description

Recombinant human OTUB2 protein is produced by our E.coli expression system and the target gene encoding Met1-His234 is expressed with a GST tag at the N-terminus.

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	E. coli
Target	OTUB2
Reactivity	Human
Protein Sequence	MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSHMSE TSFNLISEKC DILSILRDHP ENRIYRRKIE ELSKRFTAIR KTKGDGNCFY RALGYSYLES LLGKSREIFK FKERVLQTPN DLLAAGFEEH KFRNFFNAFY SVVELVEKDG SVSSLLKVFN DQSASDHIVQ FLRLLTSAFI RNRADFFRHF IDEEMDIKDF CTHEVEPMAT ECDHIQITAL SQALSIALQV EYVDEMDTAL NHHVFPEAAT PSVYLLYKTS HYNILYAADK H
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

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Storage	Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.2 μM filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, pH 7.5
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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C14orf137; OTB2; OTU2; Ubiquitin thioesterase OTUB2; Deubiquitinating enzyme OTUB2; OTU domain-containing ubiquitin aldehyde-binding protein 2; Otubain-2; Ubiquitin-specific-processing protease OTUB2

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Quick Database Links

Gene Symbol

OTUB2

UniProt

Q96DC9

NCBI Gene ID

78990

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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