Human Phospho Extracellular Signal Regulated Kinase (pERK) ELISA Kit

SKU: orb782335

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to pERK(Phospho Extracellular Signal Regulated Kinase).

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Tested Applications

ELISA

Application Notes

standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pERK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pERK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pERK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pERK in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	31.25-2000 pg/mL
Sensitivity	11.36 pg/mL
Concentration	2000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MAPK1 ELISA Kit, ERK ELISA Kit, ERK-2 ELISA Kit, ERK2 ELISA Kit, ERT1 ELISA Kit, MAPK2 ELISA Kit, P42MAPK ELISA Kit, PRKM1 ELISA Kit, PRKM2 ELISA Kit, p38 ELISA Kit, p40 ELISA Kit, p41 ELISA Kit, p41mapk ELISA Kit, p42-MAPK ELISA Kit, mitogen-activated protein kinase 1 ELISA Kit

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Human Phospho Extracellular Signal Regulated Kinase (pERK) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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