Human Poliovirus Receptor Related Protein 1 (PVRL1) ELISA Kit

SKU: orb778288

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Poliovirus Receptor Related Protein 1(PVRL1).

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PVRL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PVRL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PVRL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PVRL1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.064 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD111 ELISA Kit, HVEC ELISA Kit, PRR1 ELISA Kit, CLPED1 ELISA Kit, ED4 ELISA Kit, HIgR ELISA Kit, OFC7 ELISA Kit, PRR ELISA Kit, PVRR1 ELISA Kit, SK12 ELISA Kit, Nectin 1 ELISA Kit, Herpesvirus Entry Mediator C ELISA Kit, Herpesvirus Ig-like receptor ELISA Kit

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Human Poliovirus Receptor Related Protein 1 (PVRL1) ELISA Kit

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UniProt

Q15223

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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