Human PRDX5 protein

SKU: orb707023

Description

Recombinant Human Peroxiredoxin 5 Protein is produced by mammalian expression system and the target gene encoding Met53-Leu214 is expressed with a 6His tag at the N-terminus.

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Target	PRDX5
Reactivity	Human
Protein Sequence	HHHHHHMAPI KVGDAIPAVE VFEGEPGNKV NLAELFKGKK GVLFGVPGAF TPGCSKTHLP GFVEQAEALK AKGVQVVACL SVNDAFVTGE WGRAHKAEGK VRLLADPTGA FGKETDLLLD DSLVSIFGNR RLKRFSMVVQ DGIVKALNVE PDGTGLTCSL APNIISQL
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, pH 7.4.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ACR1; Peroxiredoxin-5, mitochondrial; Alu corepressor 1; Antioxidant enzyme B166; AOEB166; Liver tissue 2D-page spot 71B; PLP; Peroxiredoxin V; Prx-V; Peroxisomal antioxidant enzyme; TPx type VI; Thioredoxin peroxidase PMP20; Thioredoxin reductase

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Quick Database Links

Gene Symbol

PRDX5

UniProt

P30044

NCBI Gene ID

25824

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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