Human PRMT5 & MEP50 Protein, Flag,His Tag (MALS verified)

SKU: orb3158082

Description

Protein arginine methyltransferase 5 (PRMT5) is a type II enzyme that catalyzes symmetric dimethylation of arginine residues on histone and non-histone proteins. It forms a hetero-octameric complex with its essential cofactor MEP50 (methylosome protein 50), which enhances its catalytic activity and substrate selectivity. The PRMT5–MEP50 complex regulates gene expression epigenetically, influences RNA splicing, and modulates signal transduction pathways. Aberrant PRMT5–MEP50 activity is linked to tumorigenesis, cardiovascular disorders, and neurodegeneration by driving dysregulated methylation patterns that favor cell proliferation and survival.

Images & Validation

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Application Notes	The PRMT5 & MEP50 protein carries a Flag tag and a polyhistidine tag at the N-terminus. The protein has a calculated MW of 74.4 kDa & 38.3 kDa. The protein migrates as 40-43 kDa and 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	InsectCells
Target	PRMT5 & MEP50
Reactivity	Human
Tag	N-Flag \| N-10×His
Molecular Weight	74.4 kDa & 38.3 kDa
Expression Region	Ala 2 - Leu 637 & Arg 2 - Glu 342
Purity	90%
Conjugation	Unconjugated

Storage & Handling

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Storage	-70°C
Form/Appearance	Liquid
Buffer/Preservatives	50 mM Tris, 500 mM NaCl, pH 7.5
Expiration Date	6 months from date of receipt.
Dry Ice Shipping	Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
Disclaimer	For research use only

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Quick Database Links

Gene Symbol

PRMT5 & MEP50

UniProt

O14744, Q9BQA1

RefSeq:O14744, Q9BQA1

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	O14744, Q9BQA1

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