Human Procollagen II N-Terminal Propeptide (PIINP) ELISA Kit

SKU: orb776437

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen II N-Terminal Propeptide(PIINP).

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Tested Applications

ELISA

Application Notes

standard: 6000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIINP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIINP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	93.75-6000 pg/mL
Sensitivity	38.33 pg/mL
Concentration	6000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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P2NP ELISA Kit, N-Propeptide Of Type II Procollagen ELISA Kit, Procollagen II Amino Terminal Propeptide ELISA Kit

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Human Procollagen II N-Terminal Propeptide (PIINP) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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