Human Prostate Specific membrane Antigen (PSMA) ELISA Kit

SKU: orb866887

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Description

Sandwich Human PSMA ELISA Kit

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSMA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.56 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Cell growth inhibiting protein 27 ELISA Kit, Cell growth-inhibiting gene 27 protein ELISA Kit, FGCP ELISA Kit, Folate hydrolase (prostate-specific membrane antigen) 1 ELISA Kit, Folate hydrolase 1 ELISA Kit

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Human Prostate Specific membrane Antigen (PSMA) ELISA Kit

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UniProt

Q04609

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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