Human Protein S (PROS) ELISA Kit

SKU: orb776586

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein S(PROS).

Research Area

Hematology, Metabolism Research

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PROS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PROS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PROS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PROS in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Plasma
Assay Type	Sandwich
Assay Time	3.5h
Range	62.5-4000 pg/mL
Sensitivity	27.3 pg/mL
Concentration	4000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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PSA ELISA Kit, PS ELISA Kit, PS26 ELISA Kit, PS21 ELISA Kit, PS22 ELISA Kit, PS23 ELISA Kit, PS24 ELISA Kit, PS25 ELISA Kit, VKDPS ELISA Kit, VKDP-S ELISA Kit, Protein Sa ELISA Kit, Vitamin K Dependent Protein S ELISA Kit

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Quick Database Links

UniProt

P07225

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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