Human Receptor II for the Fc Region of Immunoglobulin E (FceRII) ELISA Kit

SKU: orb774970

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor II For The Fc Region Of Immunoglobulin E(FceRII).

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FceRII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FceRII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FceRII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FceRII in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.117 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD23 ELISA Kit, FCER2 ELISA Kit, BLAST-2 ELISA Kit, CLEC4J ELISA Kit, FCE2 ELISA Kit, IGEBF ELISA Kit, C-type lectin domain family 4 member J ELISA Kit, Immunoglobulin E-binding factor ELISA Kit, Lymphocyte IgE receptor ELISA Kit

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Human Receptor II for the Fc Region of Immunoglobulin E (FceRII) ELISA Kit

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UniProt

P06734

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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