Human Regenerating Islet Derived Protein 1 Beta (REG1b) ELISA Kit

SKU: orb777338

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 1 Beta(REG1b).

Research Area

Infectious Disease & Virology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human REG1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human REG1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human REG1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human REG1b in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.62 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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PSPS2 ELISA Kit, REGH ELISA Kit, REGI-BETA ELISA Kit, REGL ELISA Kit, Pancreatic Stone Protein ELISA Kit, Pancreatic Thread Protein ELISA Kit, Lithostathine 1 Beta ELISA Kit, Secretory Pancreatic Stone Protein 2 ELISA Kit

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Human Regenerating Islet Derived Protein 1 Beta (REG1b) ELISA Kit

Quick Database Links

UniProt

P48304

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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