Human Resistin (RETN) ELISA Kit

SKU: orb775090

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Resistin(RETN).

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RETN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RETN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RETN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RETN in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.058 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ADSF ELISA Kit, FIZZ3 ELISA Kit, RETN1 ELISA Kit, RSTN ELISA Kit, XCP1 ELISA Kit, Adipose tissue-specific secretory factor ELISA Kit, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein ELISA Kit, Cysteine-rich secreted FIZZ3 ELISA Kit

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

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UniProt

Q9HD89

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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ELISA (1)

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Human (1)

Amir Ghaffari Jolfayi 1, Ahmad Tashakori Beheshti 1, Seyyed Mohammadjavad Hosseini 1, Amir Akbar Fakhrabadi 1, Bahram Mohebbi 1, Mahshid Malakootian 2, Majid Maleki 2, Hamidreza Pouraliakbar 1, Saeid Hosseini 3, Maedeh Arabian Epicardial adipose tissue features as a biomarker and therapeutic target in coronary artery disease Sci Rep ., (2025)

PubMed: 40295726

Applications

ELISA

Reactivity

Human