Human Reticulon 4 (RTN4) ELISA Kit

SKU: orb778004

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Reticulon 4(RTN4).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RTN4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RTN4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RTN4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RTN4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.065 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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NSP ELISA Kit, ASY ELISA Kit, NOGO ELISA Kit, NSP-CL ELISA Kit, RTN-X ELISA Kit, Reticulon-5 ELISA Kit, Foocen ELISA Kit, Neurite outgrowth inhibitor ELISA Kit, Neuroendocrine-specific protein ELISA Kit, Neuroendocrine-specific protein C homolog ELISA Kit

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UniProt

Q9NQC3

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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