Human Sialic Acid Acetylesterase (SIAE) ELISA Kit

SKU: orb777357

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sialic Acid Acetylesterase(SIAE).

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SIAE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIAE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SIAE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SIAE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.055 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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YSG2 ELISA Kit, CSE-C ELISA Kit, LSE ELISA Kit, Sialic Acid-Specific Acetylesterase II ELISA Kit, Sialic acid-specific 9-O-acetylesterase ELISA Kit

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Human Sialic Acid Acetylesterase (SIAE) ELISA Kit

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UniProt

Q9HAT2

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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