Human Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) ELISA Kit

SKU: orb779051

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sialic Acid Binding Ig Like Lectin 7(SIGLEC7).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIGLEC7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SIGLEC7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SIGLEC7 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.058 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CDw328 ELISA Kit, CD328 ELISA Kit, p75 ELISA Kit, AIRM1 ELISA Kit, D-Siglec ELISA Kit, QA79 ELISA Kit, SIGLEC-7 ELISA Kit, P75/AIRM1 ELISA Kit, Adhesion inhibitory receptor molecule 1 ELISA Kit, QA79 membrane protein ELISA Kit

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Human Sialic Acid Binding Ig Like Lectin 7 (SIGLEC7) ELISA Kit

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UniProt

Q9Y286

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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