Human SIRPa Protein, hFc-His Tag

SKU: orb689370

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Description

Recombinant Human SIRPa with C-terminal human Fc and 6×His tag

Research Area

Epigenetics

Images & Validation

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Item 1 of 4

Key Properties

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Source	Mammalian
Expression System	HEK293
Target	SIRPa
Reactivity	Human
Tag	C-Human Fc and 6×His Tag
Molecular Weight	The protein has a predicted molecular mass of 70-98 kDa after removal of the signal peptide.
Expression Region	SIRPa(Glu31-Tyr373) hFc(Glu99-Ala330) 6×His tag
Purification	Purified from cell culture supernatant by affinity chromatography
Purity	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Form/Appearance	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Buffer/Preservatives	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Reconstitution	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SHPS1;SIRPA;CD172A;BIT;MFR;MYD1;P84;PTPNS

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Human SIRPα, hFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD47, mFc-His tagged protein orb689369 can bind its native ligand Human SIRPα, hFc-His tagged protein (orb689370) in a linear range of 3.3-26.37 ng/ml.

A: Human SIRPα, hFc-His tagged protein without freeze-thaw treatment. B: Human SIRPα, hFc-His tagged protein after one freeze-thaw cycle. C: Human SIRPα, hFc-His tagged protein after three freeze-thaw cycles. D: Human SIRPα, hFc-His tagged protein after five freeze-thaw cycles.

Lane 1: Human SIRPα, hFc-His tagged protein without freeze-thaw treatment, Lane 2: Human SIRPα, hFc-His tagged protein after one freeze-thaw cycle, Lane 3: Human SIRPα, hFc-His tagged protein after three freeze-thaw cycles, Lane 4: Human SIRPα, hFc-His tagged protein after five freeze-thaw cycles.

Quick Database Links

Gene Symbol

SIRPa

UniProt

P78324

UniProt Details

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No UniProt data available

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