Human Suppression of Tumorigenicity 14 (ST14) ELISA Kit

SKU: orb779969

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Suppression Of Tumorigenicity 14(ST14).

Research Area

Cancer Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ST14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ST14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ST14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ST14 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 ng/mL
Sensitivity	0.61 ng/mL
Concentration	100 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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SNC19 ELISA Kit, HAI ELISA Kit, MT-SP1 ELISA Kit, PRSS14 ELISA Kit, TADG15 ELISA Kit, Epithin ELISA Kit, Matriptase ELISA Kit, Prostamin ELISA Kit, Membrane-type serine protease 1 ELISA Kit, Serine protease 14 ELISA Kit, Tumor-associated differentially-expressed gene 15 ELISA Kit

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Human Suppression of Tumorigenicity 14 (ST14) ELISA Kit

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UniProt

Q9Y5Y6

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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