Human Tachykinin, Precursor 1 (TAC1) ELISA Kit

SKU: orb778892

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tachykinin, Precursor 1(TAC1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tachykinin, Precursor 1(TAC1).

Research Area

Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human TAC1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TAC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TAC1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	156.25-10000 pg/mL
Sensitivity	54.5 pg/mL
Concentration	10000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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NK2 ELISA Kit, NKNA ELISA Kit, NKA ELISA Kit, TAC2 ELISA Kit, • ELISA Kit, PPT ELISA Kit, Protachykinin-1 ELISA Kit, Substance P ELISA Kit, Neurokinin A ELISA Kit, Neuromedin L ELISA Kit, Substance K ELISA Kit, Neuropeptide K ELISA Kit, Neuropeptide gamma ELISA Kit, C-terminal-flanking peptide ELISA Kit

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Human Tachykinin, Precursor 1 (TAC1) ELISA Kit

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UniProt

P20366

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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