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Description
Research Area
Cell Biology
Images & Validation
−
Key Properties
−| Expression System | Human |
|---|---|
| Biological Activity | BLI: Loaded Biotinylated Human TGF-beta 1(Cat#orb3002438) on SA Biosensor, can bind Recombinant Human TGFBR2(Cat#orb3002421) with an affinity constant of 0.927nM as determined in BLI assay. (Regularly tested) Loaded Biotinylated Human TGF-beta 3(Cat#orb2993088) on SA Biosensor, can bind Human TGFBR2-Fc(Cat#orb3002421) with an affinity constant of 0.38 nM as determined in BLI assay. (Regularly tested). Cell Based Assay: Measured by its ability to inhibit TGF-beta 1 activity on TF‑1 human erythroleukemic cells. The ED50 for this effect is 18.41 ng/ml in the presence of 100pg/ml of recombinant human TGF-beta 1. (Regularly tested) |
| Tag | C-Fc |
| Expression Region | Thr23-Asp159 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| MW | Predicted: 42.6 KDa. Observed: 59 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TGF-beta receptor type-2; TGF-beta type II receptor;TGFBR2; Transforming growth factor-beta receptor type II
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Recombinant Human TGFBR2 Protein (orb3002421)
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