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Human TNF-alpha Protein, His Tag

SKU: orb383503
ActiveBiologically Active

Description

Human TNF-alpha Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # NP_000585.2).

Images & Validation

Application Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.3 kDa. The protein migrates as 17-19 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
TargetTNF-alpha
ReactivityHuman
TagC-6×His
Molecular Weight18.3 kDa
Expression RegionVal 77 - Leu 233
Purity95%
Endotoxins1.0 EU per μg
ConjugationUnconjugated

Storage & Handling

Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
DisclaimerFor research use only

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Human TNF-alpha Protein, His Tag

SDS-PAGE analysis of Human TNF-alpha protein

Human TNF-alpha Protein, His Tag

Humira (Adalimumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human TNF-alpha Protein, His Tag (Cat. No. orb383503) with an affinity constant of 0.244 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

UniProt Details

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Human TNF-alpha Protein, His Tag (orb383503)

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100 μg
$ 680.00
1 mg
$ 3,100.00
DispatchUsually dispatched within 5-10 working days
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