Human Transforming Growth Factor Beta Receptor I (TGFbR1) ELISA Kit

SKU: orb775305

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta Receptor I(TGFbR1).

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Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TGFbR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TGFbR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TGFbR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TGFbR1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.122 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ACVRLK4 ELISA Kit, AAT5 ELISA Kit, ALK5 ELISA Kit, SKR4 ELISA Kit, TGFR-1 ELISA Kit, Activin A Receptor Type II-Like Kinase 53kDa ELISA Kit, Activin receptor-like kinase 5 ELISA Kit, Serine/threonine-protein kinase receptor R4 ELISA Kit

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Human Transforming Growth Factor Beta Receptor I (TGFbR1) ELISA Kit

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UniProt

P36897

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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