Human Transmembrane Protease, Serine 4 (TMPRSS4) ELISA Kit

SKU: orb777594

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protease, Serine 4(TMPRSS4).

Research Area

Protein Biochemistry

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TMPRSS4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TMPRSS4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TMPRSS4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TMPRSS4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.066 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MT-SP2 ELISA Kit, CAPH2 ELISA Kit, TMPRSS3 ELISA Kit, Membrane-Type Serine Protease 2 ELISA Kit, Type II Membrane Serine Protease ELISA Kit, Channel-activating protease 2 ELISA Kit

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Human Transmembrane Protease, Serine 4 (TMPRSS4) ELISA Kit

Quick Database Links

UniProt

Q9NRS4

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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