Human Twist Transcription Factor (TWIST) ELISA Kit

SKU: orb778186

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Twist Transcription Factor(TWIST).

Research Area

Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TWIST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TWIST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TWIST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TWIST in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.061 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ACS3 ELISA Kit, BPES2 ELISA Kit, BPES3 ELISA Kit, SCS ELISA Kit, Class A basic helix-loop-helix protein 38 ELISA Kit, Acrocephalosyndactyly 3 ELISA Kit, Saethre-Chotzen Syndrome ELISA Kit, Blepharophimosis,Epicanthus Inversus And Ptosis 3 ELISA Kit

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Human Twist Transcription Factor (TWIST) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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