Human UBE2J2 protein

SKU: orb392662

Description

Recombinant human UBE2J2 protein is produced by our E. coli expression system and the target gene encoding Glu124-Arg224 is expressed with a GST tag at the N-terminus.

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	E. coli
Reactivity	Human
Protein Sequence	MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFH MEKGPTLGSI ETSDFTKRQL AVQSLAFNLK DKVFCELFPE VVEEIKQKQK AQDELSSRPQ TLPLPDVVPD GETHLVQNGI QLLNGHAPGA VPNLAGLQQA NR
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

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Storage	Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/Appearance	Liquid in a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 1mM DTT, pH 7.2.
Disclaimer	For research use only

Alternative Names

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NCUBE2; Ubiquitin-conjugating enzyme E2 J2; Non-canonical ubiquitin-conjugating enzyme 2; NCUBE-2

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Quick Database Links

UniProt

Q8N2K1

NCBI Gene ID

118424

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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