Human UBE2V1 protein

SKU: orb392671

Description

Recombinant human UBE2V1 protein is produced by our E. coli expression system and the target gene encoding Ala2-Asn147 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	E. coli
Reactivity	Human
Protein Sequence	AATTGSGVKV PRNFRLLEEL EEGQKGVGDG TVSWGLEDDE DMTLTRWTGM IIGPPRTIYE NRIYSLKIEC GPKYPEAPPF VRFVTKINMN GVNSSNGVVD PRAISVLAKW QNSYSIKVVL QELRRLMMSK ENMKLPQPPE GQCYSNLEHH HHHH
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

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Storage	Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/Appearance	Liquid in a 0.2 μM filtered solution of 50mM HEPES, 100mM NaCl, pH 8.0.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CROC1; UBE2V; UEV1; Ubiquitin-conjugating enzyme E2 variant 1; UEV-1; CROC-1; TRAF6-regulated IKK activator 1 beta Uev1A

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Quick Database Links

UniProt

Q13404

NCBI Gene ID

7335, 387522

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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