Human UBE2Z protein

SKU: orb392673

Description

Recombinant human UBE2Z protein is produced by our E. coli expression system and the target gene encoding Met1-Val246 is expressed with a 6His tag at the N-terminus.

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	E. coli
Reactivity	Human
Protein Sequence	MGSSHHHHHH SSGLVPRGSH MSIYKEPPPG MFVVPDTVDM TKIHALITGP FDTPYEGGFF LFVFRCPPDY PIHPPRVKLM TTGNNTVRFN PNFYRNGKVC LSILGTWTGP AWSPAQSISS VLISIQSLMT ENPYHNEPGF EQERHPGDSK NYNECIRHET IRVAVCDMME GKCPCPEPLR GVMEKSFLEY YDFYEVACKD RLHLQGQTMQ DPFGEKRGHF DYQSLLMRLG LIRQKVLERL HNENAEMDSD SSSSGTETDL HGSLRV
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

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Storage	Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/Appearance	Liquid in a 0.2 μM filtered solution of 50mM HEPES, 50mM NaCl, pH 8.0.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Ubiquitin-conjugating enzyme E2 Z; Uba6-specific E2 conjugating enzyme 1; Use1; Ubiquitin carrier protein Z; Ubiquitin-protein ligase Z

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Quick Database Links

UniProt

Q9H832

NCBI Gene ID

65264

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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