Human Vascular Endothelial Growth Factor B (VEGFB) ELISA Kit

SKU: orb776185

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Factor B(VEGFB).

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGFB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VEGFB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VEGFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGFB in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	5.3 pg/mL
Concentration	1000 pg/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

−

VEGF-B ELISA Kit, VEGFL ELISA Kit, VRF ELISA Kit, VEGF-Related Factor ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Human Vascular Endothelial Growth Factor B (VEGFB) ELISA Kit

Quick Database Links

UniProt

P49765

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols

Filter by Applications

ELISA (1)

Filter by Species

Human (1)

Ruonan Zhou 1 2, Yingying Xue 1 2, Ziwei Zhu 1 2, Pingyuan Xu 1 2, Lixuan Shen 1 2, Ziwei Wang 1 2, Yingying Xiang 1 2, Yue Cao 1 2, Xizhong Yu 2, Wenbin Shang VEGF-B is involved in diabetic peripheral neuropathy in patients with type 2 diabetes Growth Factors, (2024)

PubMed: 39001597

Applications

ELISA

Reactivity

Human