Human Vascular Endothelial Growth Inhibitor (VEGI) ELISA Kit

SKU: orb778919

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vascular Endothelial Growth Inhibitor(VEGI).

Research Area

Cardiovascular Research; Pharmacology & Drug Discovery

Images & Validation

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Item 1 of 1

Tested Applications	ELISA

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	62.5-4000 pg/mL
Sensitivity	27.1 pg/mL

Procedure & Performance

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Assay Principle	The kit is based on a sandwich enzyme immunoassay principle. The microtiter plate is pre-coated with a capture antibody specific to the target analyte. Standards or samples are added to the wells, followed by a biotin-conjugated detection antibody specific for the analyte. Avidin conjugated to horseradish peroxidase (HRP) is then added and incubated. After addition of the TMB substrate, color develops only in wells containing the analyte bound to the detection antibody and HRP–avidin complex. The reaction is stopped with an acidic solution, and absorbance is measured at 450 nm ± 10 nm. The analyte concentration in the samples is determined by comparison with a standard curve. Read more...
Kit Components	1. ELISA Microplate 2. Standards 3. Detection Antibody 4. HRP-Streptavidin Conjugate 5. TMB Substrate 6. Dilution buffers 7. Stop Solution 8. Wash Buffer 9. Plate Sealers 10. Manual Read more...
Reagent Preparation	1. Wash Buffer: Prepare the 1X Wash Buffer using distilled water according to the manual. 2. Standard: Perform gradient dilution according to the instructions in the manual. 3. Other Concentrated Reagents: Dilute the concentrated reagents using the Dilution Buffers provided in the kit to 1 X working solutions as instructed in the manual. Always use a clean pipette tip for each different solution. Read more...
Assay Procedure	This procedure is for reference only. 1. After the kit equilibrates to room temperature, add standards or samples to each well and incubate. 2. Discard liquid, add wash buffer to each well, wash the plate three times, and blot dry on clean absorbent paper. 3. Add biotinylated antibody working solution to each well and incubate. 4. Discard liquid, add wash buffer to each well, wash the plate three times, and blot dry on clean absorbent paper. 5. Add streptavidin-HRP working solution to each well and incubate._x000b_6. Discard liquid, add wash buffer to each well, wash the plate five times, and blot dry on clean absorbent paper. 7. Add TMB substrate solution to each well and incubate in the dark. 8. Add stop solution to each well, mix thoroughly, and immediately read OD at 450 nm. Read more...
Materials Required	1. Microplate readers 2. Centrifuge 3. Incubator 4. Automated plate washer 5. Single-channel or multi-channel high-precision pipettes 6. Disposable pipette tips 7. Sterile tubes 8. Eppendorf tubes 9. Absorbent paper 10. Loading slots Read more...
Precision	Intra-assay Precision (Precision within an assay): CV% < 8% Intra-assay precision was evaluated by testing multiple replicates of samples within the same plate. Inter-assay Precision (precision between assays): CV% < 10% Inter-assay precision was evaluated by testing samples across different plates. Read more...
Calculation of Results	1. Average the duplicate readings for each Standard, Control, and Sample, and subtract the mean optical density of the zero Standard. 2. Construct a standard curve by plotting the target concentration on the y-axis against absorbance on the x-axis and draw a curve through the data points. 3. Determine the sample concentration by substituting the OD450 value into the standard curve. For diluted samples, multiply the calculated value by the corresponding dilution factor. Read more...
Curve Fitting Softwares	1. Curve Expert 2. Thermo SkanIt RE 3. SciDAVis 4. LabPlot 5. …… Read more...

Customer Validated Data

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The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.

Success by Application

Reactivity Distribution

Latest Experiments

2 Results

Dilution

Sample

Summary

ELISA

Human

Stool (fecal) samples

The Human Interleukin 23 (IL23) ELISA...

ELISA

Human

Saliva

The Human Interleukin 23 (IL23) ELISA...

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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TNFSF15 ELISA Kit, TL1 ELISA Kit, TL1A ELISA Kit, VEGI192A ELISA Kit, Tumor Necrosis Factor(ligand)Superfamily,Member 15 ELISA Kit, TNF Superfamily Ligand TL1A ELISA Kit, TNF Ligand-Related Molecule 1 ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Human Vascular Endothelial Growth Inhibitor (VEGI) ELISA Kit

Standard curve for orb778919 ELISA kit.

Quick Database Links

UniProt

O95150

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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