Human VEGF165 Mouse Monoclonal Antibody

SKU: orb1152042

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Description

Research Area

Angiogenesis, Angiogenesis and Vasculogenesis, Angiogenic factors, Cancer Research, Cell Biology, Growth Factors, Growth Factors and Hormones, Hypoxia, SARS /COVID-19, Varscular, Varscular System

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, WB
Dilution Range	WB=1:500-2000, ELISA=1:5000-10000
Reactivity	Human
Predicted Reactivity	Human

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	19F2
Immunogen	Recombinant human VEGF165 Protein (27-191/191aa)
Target	VEGFA
Molecular Weight	18 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS (pH7.4).
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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VEGF165; Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; Vegf; VEGFA; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGF A Precursor.

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Measured by its binding ability in a indirect ELISA. Immobilized Human VEGF165, His Tag at 2 µg/mL (100 µl/well) can bind Anti-Human VEGF165 monoclonal antibody, the minimum detection concentration is 0.5 ng/ml.

Sample: Lane 1: Recombinant Human VEGF165 Protein at 500 ng, Primary: Mouse Anti-VEGF165 Monoclonal Antibody at 1/1000 dilution (Cat.orb1152042), Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 21kD, Observed band size: 25kD.

Quick Database Links

Gene Symbol

VEGFA

UniProt

P15692

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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