Cart summary

You have no items in your shopping cart.

ICSN3250 HCl

SKU: orb2944188

Description

ICSN3250 HCl is a selective mTOR inhibitor that competitively binds the FRB domain, displacing phosphatidic acid to block mTOR activation and induce cancer cell death. It is a research tool for studying mTOR signaling in oncology, applicable in both in vitro cell-based assays and in vivo xenograft models.

Research Area

Cell Biology, Signal Transduction

Images & Validation

Key Properties

CAS Number1561902-79-1
MW633.13
Purity98.46%
FormulaC31H41ClN4O8
SMILESCl.O=N(=O)C=1C=C(C=C(O)C1O)C2=CN(C=C2C=3C=C(O)C(O)=C(C3)N(=O)=O)CCCN4CCCCCCCCCCCC4
Targetp62,Autophagy,mTOR,ATG,S6 Kinase

Bioactivity

In Vivo
ICSN3250 HCl (100 nmol/L, 72 h) was applied to primary colorectal cancer cells and matched fibroblasts isolated from patient tumor tissue. Cell viability and morphology were assessed microscopically. ICSN3250 HCl selectively reduced cancer cell viability without affecting normal fibroblasts.
In Vitro
ICSN3250 HCl was tested on multiple cancer cell lines (HCT116, U2OS, U87, K562) and normal cells (HUVEC, NHDF, HFDPC) under standard culture conditions (10–100 nmol/L, 24–72 h). Western blot, autophagy markers, and viability assays were used to assess activity. Results showed ICSN3250 HCl selective mTORC1 inhibition and cytotoxicity in cancer cells with minimal toxicity to normal cells.

Storage & Handling

Storagestore at low temperature,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ICSN 3250 HCl, ICSN3250 HCl(1561902-73-5 Free base)
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Key Properties

No computed properties available.

Documents Download

Datasheet
Product Information
Download

Request a Document

Protocol Information

ICSN3250 HCl (orb2944188)

  • Star
  • Star
  • Star
  • Star
  • Star
  • 0.0
Based on 0 reviews

Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.

Login to Submit a Review

No reviews yet

Step 1: Enter information below

(Recommended: An additional animal making an allowance for loss during the experiment)

Step 2: Enter the in vivo formulation

(This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO +
%+
% Tween 80 +
%

Available Sizes

Select a size below

1 mg
$ 240.00
5 mg
$ 530.00
10 mg
$ 740.00
25 mg
$ 1,120.00
50 mg
$ 1,500.00
100 mg
$ 2,010.00
DispatchUsually dispatched within 5-10 working days
Bulk Enquiry