Recombinant Insulin-like Growth Factor 1 Antibody / IGF-1

SKU: orb2643722

Description

This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

Images & Validation

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Tested Applications	FACS
Dilution Range	Flow cytometry: 1-2ug/10^6 cells in 0.1ml
Reactivity	Human, Mouse, Rat
Application Notes	Optimal dilution of the recombinant Insulin-like Growth Factor 1 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG
Clone No.	IGF1/2872R
Immunogen	Recombinant full-length human protein was used as the immunogen for the recombinant Insulin-like Growth Factor 1 antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant Insulin-like Growth Factor 1 Antibody / IGF-1

Flow cytometry testing of human K562 cells with recombinant Insulin-like Growth Factor 1 antibody (clone IGF1/2872R); Red=isotype control, Blue= recombinant Insulin-like Growth Factor 1 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant Insulin-like Growth Factor 1 antibody (clone IGF1/2872R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P05019

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No UniProt data available

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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