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Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 3
| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB - 1:2000, FC - 1:25 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Porcine |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | BTD42691 |
| Immunogen | This IGFBP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-92 amino acids from the N-terminal region of human IGFBP4. Antigen Region: 66-92 aa. |
| Target | IGFBP4 |
| Molecular Weight | 27934 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Insulin-like growth factor-binding protein 4, IBP-4, IGF-binding protein 4, IGFBP-4, IGFBP4, IBP4
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Overlay histogram showing A549 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
Anti-IGFBP4 Antibody (N-term) at 1:2000 dilution + Rat ovary lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-IGFBP4 Antibody (N-term) at 1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Mouse ovary lysate. Lane 3: Rat ovary lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001543.2 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IGFBP4 Antibody (N-term) (orb1929696)
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