IL-11 Rabbit Polyclonal Antibody

SKU: orb500753

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Description

Images & Validation

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Item 1 of 4

Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Mouse, Rat
Predicted Reactivity	Bovine, Canine, Guinea pig, Human, Porcine

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human IL-11 (22-120/199aa)
Target	IL11
Molecular Weight	19 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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Interleukin-11; Adipogenesis inhibitory factor; AGIF; IL 11; IL11; Interleukin 11; Oprelvekin; IL11_HUMAN.

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Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (IL-11) Polyclonal Antibody, Unconjugated (orb500753) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (IL-11) Polyclonal Antibody, Unconjugated (orb500753) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Rat Kidney tissue lysates, Primary: Anti-IL-11 (orb500753) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 19 kDa, Observed band size: 19 kDa.

Sample: Testis (Mouse) Lysate at 30 ug, Primary: Anti-IL-11 (orb500753) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution, Predicted band size: 19 kD, Observed band size: 19 kD.

Quick Database Links

Gene Symbol

IL11

UniProt

P20809

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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