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IL21 Antibody (Biotin)

SKU: orb1272761

Description

IL21 Antibody (Biotin)

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman
Application Notes
ELISA:Sandwich:To detect hIL-21 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IL-21 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-21. Western Blot:To detect hIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
ImmunogenProduced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-21. Human IL-21 specific antibody was purified by affinity chromatography and then biotinylated.
TargetIL21
ConjugationBiotin

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Za11, IL-21Interleukin-21, Za11, IL-21

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqQ9HBE4

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Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

IL21 Antibody (Biotin) (orb1272761)

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0.05 mg
$ 780.00
DispatchUsually dispatched within 5-10 working days
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