IL21 Antibody

Immunohistochemistry Validation of IL-21 in Human Tonsil. Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IL-21 antibody (orb1239478) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of IL-21 in Mouse Spleen. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL-21 antibody (orb1239478) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of IL-21 in Rat Spleen. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-IL-21 antibody (orb1239478) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Induced Expression Validation of IL-21 expresssion in patients with Crohn's Disease. Enhanced IL-21 was observed in involved but not uninvolved CD, and was not associated with any CD phenotype, such as fibrostenosing disease. IL-21 expression was detected by anti-IL-21 antibodies (orb1239478).

Induced Expression Validation of IL-21 expresssion in patients with Helicobacter Pylori (Hp). (A) shows IL-21 expression levels from biopsies of patients with Hp infection and without Hp infection (n=3). (C) shows IL-21 expression levels in CD3+LPMC cells from Hp-positive and Hp-negative patients (n=3). Both WB show IL-21 expression increased in patients with Hp infection.

Regulation of IL-21 expresssion in duodenal biopsies of two patients with active Celiac disease (ACD). (b) shows IL-21 expression levels treated with dimethyl sulfoxide (DMSO) or wortmannin (WRT). (c) shows IL-21 expression levels with the inhibition of IL-15 antibody (aIL-15). IgG isotype was used as a control. Both WB show IL-21 expression decreased with the treatment with WRT or the inhibition of IL-15 antibody.

Western Blot Validation in Human, Mouse and Rat Tissues. Loading: 15 µg of lysates per lane. Antibodies: IL-21 orb1239478, (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-21 recombinant protein per lane. Antibodies: IL-21 orb1239478 (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.