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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide of human IL1B |
| Target | IL1B |
| Molecular Weight | Observed: 17kDa, 31kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−IL1B, interleukin 1, beta, IL-1, IL1-BETA, IL1F2, IL-1 beta, catabolin, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
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Western blot analysis of extracts of various cell lines, using IL1B antibody (orb1259070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Western blot analysis of extracts of THP-1 cells, using IL1B antibody (orb1259070) at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) at 37 ℃ for overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry of paraffin-embedded rat lung using IL1B antibody (orb1259070) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of THP-1 cells using IL1B antibody (orb1259070) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse lung using IL1B antibody (orb1259070) at dilution of 1:100. Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
IL1B
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IL1B Antibody (orb1259070)
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