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IL1RL2 Antibody (Center)

SKU: orb1936721

Description

IL1RL2 Antibody (Center)

Images & Validation

Tested ApplicationsFC, WB
Dilution rangeWB - 1:8000, FC - 1:25
ReactivityHuman

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
Molecular Weight65405 Da
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
DisclaimerFor research use only

Alternative Names

IL1RRP2
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

IL1RL2 Antibody (Center)

All lanes: Anti-IL1RL2 Antibody (Center) at 1:8000 dilution. Lane 1: HL-60 whole cell lysate. Lane 2: U-87 MG whole cell lysate. Lane 3: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

IL1RL2 Antibody (Center)

Overlay histogram showing K562 cells (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol

IL1RL2 Antibody (Center) (orb1936721)

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Available Sizes

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50 μl
$ 140.00
100 μl
$ 240.00
DispatchUsually dispatched within 5-10 working days
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