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IL2RG Antibody (N-term)
SKU: orb1927910
Description
Research Area
Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | FC, IF, WB |
|---|---|
| Dilution Range | WB - 1:2000 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 42287 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Quality Guarantee
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Western blot analysis of IL2RG Antibody (N-term) in HL-60 cell line lysates (35 ug/lane). IL2RG (arrow) was detected using the purified Pab.
IL2RG Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from Jurkat cell line, using IL2RG Antibody (N-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human spleen tissue labeling IL2RG at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on human spleen tissue. The nuclear counter stain is DAPI (blue).
All lanes: Anti-IL2RG Antibody (N-term) at 1:2000 dilution. Lane 1: human spleen lysates. Lane 2: Jurkat whole cell lysates. Lane 3: Daudi whole cell lysates. Lane 4: KG-1 whole cell lysates. Lane 5: MOLT-4 whole cell lysates. Lane 6: HL-60 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
IL2RG Antibody (N-term) (orb1927910)
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