CD123 Antibody / IL3RA

SKU: orb2642154

Description

CD123 is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudo-autosomal region on chromosomes X or Y.

Images & Validation

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Tested Applications	IHC-P
Dilution range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Human
Application Notes	Optimal dilution of the CD123 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2b, kappa
Clone No.	IL3RA/1531
Immunogen	A portion of amino acids 26-171 was used as the immunogen for the CD123 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human lymph node with CD123 antibody. HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9 for 10-20 min and allow to cool.

IHC staining of FFPE human tonsil with CD123 antibody. HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9 for 10-20 min and allow to cool.

IHC staining of FFPE human gastric carcinoma with CD123 antibody. HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9 for 10-20 min and allow to cool.

SDS-PAGE analysis of purified, BSA-free CD123 antibody (clone IL3RA/1531) as confirmation of integrity and purity.

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UniProt

P26951

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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