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IL5RA Antibody (N-term)
SKU: orb1930605
Description
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P, WB |
|---|---|
| Dilution range | WB - 1:1000, IHC-P - 1:100-500, FC - 1:10-50 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 47685 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−IL5R
Quality Guarantee
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IL5RA Antibody (N-term) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL5RA Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL5RA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of lysate from MDA-MB-453 cell line, using IL5RA Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug per lane.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IL5RA Antibody (N-term) (orb1930605)
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