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Integrin β3 rabbit pAb Antibody
SKU: orb765505
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Description
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Integrin beta3. AA range:739-788 |
| Molecular Weight | 110 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-ITGB3 antibody, anti-GP3A antibody, anti-Integrin beta-3 antibody, anti-Platelet membrane glycoprotein IIIa antibody, anti-GPIIIa antibody, anti-CD antigen CD61 antibody
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Immunofluorescence analysis of A549. 1, primary Antibody (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.
Western Blot analysis of various cells using Integrin β3 Polyclonal Antibody diluted at 1:500.
Western Blot analysis of NIH-3T3 cells using Integrin β3 Polyclonal Antibody diluted at 1:500.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Integrin beta3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Integrin β3 rabbit pAb Antibody (orb765505)
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