Recombinant ITGAE Antibody / Integrin alpha E / CD103

SKU: orb2644354

Description

This MAb recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E beta-7 (aEb7) integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. Antibody to CD103 is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL).

Images & Validation

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Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant ITGAE antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rITGAE/2063
Immunogen	Recombinant human ITGAE partial protein was used as the immunogen for the recombinant ITGAE antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Recombinant ITGAE Antibody / Integrin alpha E / CD103

IHC staining of FFPE human tonsil with recombinant ITGAE antibody (clone rITGAE/2063). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant ITGAE antibody (clone rITGAE/2063) as confirmation of integrity and purity.

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UniProt

P38570

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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