Interferon gamma (IFNG) Antibody

SKU: orb751169

Description

Mouse monoclonal antibody against IFN gamma conjugated to Biotin

Images & Validation

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Item 1 of 4

Tested Applications	FC, IF, IHC
Dilution range	Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Reactivity	Human
Application Notes	Positive Control: PMA stimulated human peripheral blood mononuclear cells. Renal or testicular carcinoma

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone No.	IFNG/466
Immunogen	Purified recombinant human full-length IFNG protein
Target	IFNG
Source/Expression System	Bioreactor
Molecular Weight	20-25kDa
Purification	Protein A
Conjugation	Biotin

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Buffer/Preservatives	Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
Concentration	Purified Ab conjugated to Biotin
Disclaimer	For research use only

Alternative Names

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Anti-AMCF1 antibody, anti-Beta thromboglobulin like protein antibody, anti-CXC chemokine ligand 8 (CXCL8) antibody, anti-Emoctakin antibody, anti-Granulocyte chemotactic protein 1 (GCP1) antibody, anti-IFG antibody, anti-IFI antibody, anti-IFN Immune antibody, anti-IFN-gamma (IFNG) antibody, anti-Interleukin 8 antibody, anti-Interferon gamma antibody, anti-LECT antibody, anti-LUCT antibody, anti-Lymphocyte derived neutrophil activating factor antibody, anti-LYNAP antibody, anti-Macrophage Activating Factor (MAF) antibody, anti-Monocyte derived neutrophil activating protein (MONAP) antibody, anti-Monocyte derived neutrophil chemotactic factor (MDNCF) antibody, anti-Neutrophil activating factor (NAF) antibody, anti-Neutrophil activating peptide 1 (NAP1) antibody, anti-Neutrophil activating protein 1 (NAP1) antibody, anti-SCYB8 antibody, anti-T cell chemotactic factor antibody, anti-T Cell Interferon antibody

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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Interferon gamma Mouse Monoclonal Antibody (IFNG/466).

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Interferon gamma Mouse Monoclonal Antibody (IFNG/466).

SDS-PAGE Analysis Purified Interferon gamma Mouse Monoclonal Antibody (IFNG/466). Confirmation of Purity and Integrity of Antibody.

Quick Database Links

Gene Symbol

IFNG

UniProt

P01579

NCBI Gene ID

3458

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

IHC

Immunohistochemistry

Flow Cytometry

Immunofluorescence