IL18/IL1F4 Human Monoclonal Antibody

SKU: orb2960417

Description

InVivoMAb Anti-Human IL18/IL1F4 (BKX2252) is a human monoclonal antibody detecting IFN-gamma-inducing factor in ELISA, FCM, Neutralization. Suitable for Human. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 5

Tested Applications	ELISA, FC, NeA
Reactivity	Human

Key Properties

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Antibody Type	Recombinant Antibody
Host	Human
Clonality	Monoclonal
Isotype	IgG1, kappa
Clone No.	BKX2252
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin	Please contact with the lab for this information.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS, pH 7.4.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IFN-gamma-inducing factor, IL1F4, Interleukin-1 gamma, IL-18, IL-1 gamma, Interleukin-18, Interferon gamma-inducing factor, IGIF, IL18, Iboctadekin

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Flow-cytometry using anti-human IL18 antibody. HaCaT cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL18 antibody monoclonal antibody (Catalog # orb2960417, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human IL18 antibody. HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL18 antibody monoclonal antibody (Catalog # orb2960417, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human IL18 antibody. PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL18 antibody monoclonal antibody (Catalog # orb2960417, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human IL18 antibody. THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL18 antibody monoclonal antibody (Catalog # orb2960417, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for InVivoMAb Anti-Human IL18/IL1F4.

Quick Database Links

UniProt

Q14116

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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