IL6 Human Monoclonal Antibody

SKU: orb2960425

Description

IL6 Human Monoclonal Antibody is an unconjugated monoclonal antibody. It shows reactivity with Human samples. It is suitable for use in ELISA, FC, NeA applications. The antibody is supplied in liquid form. It is Protein A/G purified from cell culture supernatant. Purity is >95% as determined by SDS-PAGE.

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, NeA
Reactivity	Human

Key Properties

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Antibody Type	Recombinant Antibody
Host	Human
Clonality	Monoclonal
Isotype	IgG1, kappa
Clone No.	BKX2244
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin	Please contact with the lab for this information.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS, pH 7.4.
Concentration	Lot dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Hybridoma growth factor, BSF-2, IFN-beta-2, CDF, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, Interferon beta-2, IFNB2, CTL differentiation factor

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Flow-cytometry using anti-human IL6 antibody. IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # orb2960425, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human IL6 antibody. LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # orb2960425, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE for InVivoMAb Anti-Human IL6.

Western blot analysis was performed using anti-IL6 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human IL6 (Catalog No: orb2969496), 50 ng, Lane 2: recombinant human IL6 (Catalog No: orb2969496), 30 ng, Lane 3: recombinant human IL6 (Catalog No: orb2969496), 20 ng, Lane 4: recombinant human IL6 (Catalog No: orb2969496), 10 ng.

Quick Database Links

UniProt

P05231

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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