IPAF/NLRC4 Antibody

SKU: orb1538540

Description

IPAF/NLRC4 Antibody

Images & Validation

−

Item 1 of 2

Tested Applications	ELISA, ICC, IF, IHC, IHC-P, WB
Dilution Range	ELISA, ICC, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 2 µg/ml)
Reactivity	Human
Application Notes	Further information: Ipaf polyclonal antibody can be used for the detection of Ipaf by Western blot at 1 and 2 ug/mL. A 110 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: 110 kDa Read more...

Key Properties

−

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic peptide corresponding to amino acids near the C-Terminus of human Ipaf. The immunogen is located within the last 50 amino acids of Ipaf.
Target	IPAF / NLRC4
Purification	Peptide Column Affinity Chromatography
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS containing 0.02% Sodium Azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

NLRC4, CARD12, CLANA, CLANB, CLANC, CLAND, CLAN, CLR2.1, Clan protein, CLAN1, Ice protease-activating factor, NOD-like receptor C4, ICE-protease activating factor, IPAF

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Immunofluorescence of Ipaf in THP1 cells with Ipaf antibody at 10 ug/ml.

Western blot analysis of Anti-IPAF antibody (orb1538540, 2 µg/ml; 15 µg of lysate per lane). Lane 1: THP-1 Cell lysate. Antibody produced band at ~116 kDa.

Quick Database Links

Gene Symbol

IPAF / NLRC4

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC

Immunohistochemistry

View Protocol

IHC-P

Immunohistochemistry Paraffin

View Protocol

Immunofluorescence

View Protocol

ICC

Immunocytochemistry

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols