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ITGA2 Antibody

SKU: orb1274426
FeaturedFeatured Product

Description

ITGA2 Antibody

Images & Validation

Tested ApplicationsIF, IHC, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein of human ITGA2
TargetITGA2
Molecular WeightObserved: 150kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid
Concentrationbatch dependent
DisclaimerFor research use only

Alternative Names

ITGA2, Alpha2 integrin, BR, CD49b antigen, CD49B, Integrin alpha2, Integrin Alpha 2, Integrin alpha-2, HPA-5, VLA-2, VLAA2, VLA-2 subunit alpha, Collagen receptor, GPIa, Platelet antigen Br, Platelet glycoprotein GPIa, VLA2 receptor, alpha-2 subunit

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ITGA2 Antibody

Western blot analysis of extracts of various cell lines, using ITGA2 antibody (orb1274426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

ITGA2 Antibody

Western blot analysis of extracts from normal (control) and ITGA2 knockout (KO) HeLa cells, using ITGA2 antibody (orb1274426) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

ITGA2 Antibody

Immunohistochemistry of paraffin-embedded rat brain using ITGA2 Antibody (orb1274426) at dilution of 1:100 (40x lens).

ITGA2 Antibody

Immunohistochemistry of paraffin-embedded mouse brain using ITGA2 Antibody (orb1274426) at dilution of 1:100 (40x lens).

ITGA2 Antibody

Immunofluorescence analysis of U2OS cells using ITGA2 antibody (orb1274426) at dilution of 1:100. Blue: DAPI for nuclear staining.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol

ITGA2 Antibody (orb1274426)

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Available Sizes

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100 μl
$ 960.00
DispatchUsually dispatched within 5-10 working days
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