JHDM1A/KDM2A Antibody

SKU: orb1539204

Description

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, IHC, IHC-P, WB
Dilution range	IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:32000), WB (0.5 - 2 µg/ml)
Reactivity	Canine, Equine, Hamster, Human, Mammal, Monkey, Porcine, Rabbit, Rat
Application Notes	Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 140kD band observed in Human Uterus lysates (calculated MW of 133kD according to NP_036440.1). Recommended concentration: 0.5-2 ug/ml.

Key Properties

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Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence C-PRKDRQVHLTHFELE, from the internal region of the protein sequence according to NP_036440.1.
Target	JHDM1A / KDM2A
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration	0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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KDM2A, CXXC8, FBL11, DKFZP434M1735, JHDM1A, F-box protein Fbl7, F-box/LRR-repeat protein 11, KIAA1004, FBXL11, Lysine-specific demethylase 2A, LILINA, SF-KDM2A, F-box protein Lilina, FBL7

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Anti-JHDM1A / KDM2A antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

KDM2A antibody (0.5 ug/ml) staining of Human Uterus lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

JHDM1A / KDM2A

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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IHC-P

Immunohistochemistry Paraffin

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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